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F(ab')2 Rat IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

F(ab')2 Rat IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Catalog Number: orb348344

DispatchUsually dispatched within 5-10 working days
$ 480.00
Catalog Numberorb348344
CategoryAntibodies
DescriptionF(ab')2 Rat IgG (H&L) antibody (Peroxidase)
ClonalityPolyclonal
Species/HostGoat
IsotypeIgG F(ab')2
ConjugationHRP
ReactivityRat
Form/AppearanceLyophilized
Concentration1.0 mg/mL
Buffer/Preservatives0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
ImmunogenAnti-Rat IgG was produced by repeated immunization with rat IgG whole molecule in goat.
Tested applicationsELISA, IHC, WB
Dilution rangeELISA: 1:100,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:10,000
Application notesAnti-Rat IgG (H&L) peroxidase conjugated Antibody has been tested by ELISA and is assayed against 1.0 ug of Rat IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 ug of Rat IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
Antibody TypeSecondary Antibody
StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Alternative namesGoat F(ab')2 Anti-Rat IgG Peroxidase Conjugated An
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NoteFor research use only