F(ab')2 Rat IgG (H&L) Antibody Fluorescein Conjugated

SKU: orb346792

Description

F(ab')2 Rat IgG (H&L) antibody (FITC)

Images & Validation

• Tested Applications: FC, FLISA, IF
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Reactivity: Rat
• Application Notes:
  Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG F(ab')2
• Immunogen: Rat IgG whole molecule
• Purity: This product is a F(ab’)2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Goat IgG F(c) or anti-Pepsin.

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Thimerosal
• Concentration: 10.0 mg/mL
• Disclaimer: For research use only

Alternative Names

goat F(ab')2 Anti-Rat IgG fluorescein conjugated Antibody, goat F(ab')2 Anti-Rat IgG FITC conjugated Antibody, goat Fab2 Anti-Rat IgG antibody fluorescein conjugation