F(ab')2 Rat IgG Fc Antibody Pre-Adsorbed

• Catalog Number: orb348339
• Category: Antibodies
• Description: F(ab')2 Rat IgG F(c) antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Rat
• Isotype: IgG F(ab')2
• Immunogen: Anti-Rat IgG F(c) was produced by repeated immunization with Rat F(c) fragment in goat.
• Antibody Type: Secondary Antibody
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:10,000 - 1:50,000, IHC: 1:1,000 - 1:3,000, WB: 1:2,000 - 1:5,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Rat F(c) antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(ab) or Bovine, Horse and Human Serum Proteins.
• Conjugation: Unconjugated
• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Goat F(ab')2 Anti-Rat IgG F(c) Antibody, Goat Fab2
• Note: For research use only
• Application notes: F(ab')2 Anti-Rat IgG F(c) Antibody has been tested by SDS=PAGE and is suitable for immunoblotting, western blot, dot blot, ELISA, and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
• Expiration Date: 12 months from date of receipt.

SDS-PAGE results of Goat F(ab')2 Anti-Rat IgG F(c) Antibody. Lane 1: reduced Goat F(ab')2 anti-Rat. Lane 2: Opal Prestained Ladder. Lane 3: non-reduced Goat F(ab')2 anti-Rat. Load: 1.0ug. 4-20% Lonza SDS-PAGE; Coomassie Stained.