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F(ab')2 Rabbit IgG (H&L) Antibody Biotin Conjugated
SKU: orb348298
Description
Images & Validation
−
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution range | ELISA: 1:142,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000 |
| Reactivity | Rabbit |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG F(ab')2 |
| Immunogen | Rabbit IgG whole molecule |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.0 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−Goat F(ab')2 Anti-Rabbit IgG Biotin Conjugated Antibody, Goat Fab2 Anti-Rabbit IgG Antibody Biotin Conjugation
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
F(ab')2 Rabbit IgG (H&L) Antibody Biotin Conjugated (orb348298)
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