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F(ab')2 Dog IgG (H&L) Antibody Biotin Conjugated
Catalog Number: orb348103
| Catalog Number | orb348103 |
|---|---|
| Category | Antibodies |
| Description | F(ab')2 Dog IgG (H&L) antibody (Biotin) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC, WB |
| Reactivity | Canine |
| Isotype | IgG F(ab')2 |
| Immunogen | Dog IgG whole molecule |
| Antibody Type | Secondary Antibody |
| Concentration | 1 mg/mL |
| Dilution range | ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000 |
| Form/Appearance | Lyophilized |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Dog IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum, Dog IgG and Dog Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c). |
| Conjugation | Biotin |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Alternative names | Rabbit F(ab')2 Anti-Dog Biotin Conjugated Antibody Read more... |
| Note | For research use only |
| Application notes | F(ab')2 Anti-Dog IgG Biotin Antibody has been tested by ELISA and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. |
| Expiration Date | 12 months from date of receipt. |
ELISA results of purified Rabbit F(ab')2 Anti-DOG IgG Antibody Biotin Conjugated tested against purified Dog IgG. Each well was coated in duplicate with 1.0 µg of Dog IgG (Blue Line). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer, Streptavidin-HRP conjugated (p/n orb348767), and TMB substrate (p/n orb348651).
