Cart summary

You have no items in your shopping cart.

F(ab')2 Goat IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Catalog Number: orb348124

DispatchUsually dispatched within 5-10 working days
$ 440.00
Catalog Numberorb348124
CategoryAntibodies
DescriptionF(ab')2 Goat IgG (H&L) antibody (Peroxidase)
ClonalityPolyclonal
Species/HostDonkey
IsotypeIgG F(ab')2
ConjugationHRP
ReactivityGoat
Form/AppearanceLyophilized
Concentration1.0 mg/mL
Buffer/Preservatives0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Goat IgG and Goat Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Rat Serum Proteins.
ImmunogenGoat IgG whole molecule
Tested applicationsELISA, IHC, WB
Dilution rangeELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:5,000, WB: 1:2,000 - 1:10,000
Application notesF(ab')2 Anti-Goat IgG Peroxidase Antibody has been tested by ELISA and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Antibody TypeSecondary Antibody
StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Alternative namesDonkey F(ab')2 Anti-Goat IgG Antibody Peroxidase C
Read more...
NoteFor research use only
F(ab')2 Goat IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

ELISA Results of F(ab')2 Donkey Anti-Goat IgG Peroxidase Conjugated Min X Ch, GP, Ham, Hs, Hu, Ms, Rb, Rt Serum Proteins. Each well was coated in duplicate with 1.0 µg of Goat IgG (p/n orb2652767). The working dilution is 267000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS, and TMB substrate (p/n orb348651).