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F(ab')2 Goat IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

F(ab')2 Goat IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Catalog Number: orb348117

DispatchUsually dispatched within 5-10 working days
$ 440.00
Catalog Numberorb348117
CategoryAntibodies
DescriptionF(ab')2 Goat IgG (H&L) antibody (Peroxidase)
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG F(ab')2
ConjugationHRP
ReactivityGoat
Form/AppearanceLyophilized
Concentration1.0 mg/mL
Buffer/Preservatives0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
ImmunogenAnti-Goat IgG whole molecule was produced by repeated immunization with Goat IgG whole molecule in rabbit.
Tested applicationsELISA, IHC, WB
Dilution rangeELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
Application notesF(ab')2 Anti-Goat IgG Peroxidase Antibody has been tested by ELISA and is assayed against 1.0 ug of Goat IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 is suggested for this product. This secondary conjugated antibody anti-goat is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Antibody TypeSecondary Antibody
StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Alternative namesRabbit F(ab')2 Anti-Goat IgG Antibody peroxidase C
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NoteFor research use only