Fab Rat IgG (H&L) Antibody

• Catalog Number: orb348495
• Category: Antibodies
• Description: Fab Rat IgG (H&L) antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IHC, WB
• Reactivity: Rat
• Isotype: IgG Fab
• Immunogen: Rat IgG whole molecule
• Antibody Type: Secondary Antibody
• Concentration: 2.0 mg/mL
• Dilution range: ELISA: 1:5,000 - 1:20,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
• Conjugation: Unconjugated
• Storage: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Goat Fab Anti-Rat IgG Antibody, Goat Fab Fragment
• Note: For research use only
• Application notes: Fab Anti-Rat IgG (H&L) Antibody has been tested by ELISA, SDS-PAGE, and dot blot and is suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
• Expiration Date: 12 months from date of receipt.

Dot Blot of Fab Goat Anti-Rat IgG Antibody. Rat IgG (p/n orb2652744) (1) 100 ng, (2) 33.33 ng, (3) 11.11 ng, (4) 3.70 ng, (5) 1.23 ng. Primary Antibody: Fab Goat Anti-Rat IgG at 1.0 µg/ml for 1hr at RT. Secondary Antibody: Donkey Anti-Goat IgG HRP (p/n orb347032) at 1:40000 for 30 mins at RT. Block: BlockOut buffer (p/n orb348644) for 30 mins at RT. Exposure: 5 sec.