Fab Rat IgG (H&L) Antibody Peroxidase Conjugated

SKU: orb348497

Description

Fab Rat IgG (H&L) antibody (Peroxidase)

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:10,000 - 1:40,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:4,000
• Reactivity: Rat
• Application Notes:
  Anti-Rat IgG HRP antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG Fab
• Immunogen: Anti-Rat IgG (H&L) was produced by repeated immunization with rat IgG whole molecule in goat.
• Purity: Anti-Rat IgG (H&L) peroxidase conjugated antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Concentration: 1.0 mg/mL
• Disclaimer: For research use only

Alternative Names

Goat Fab Anti-Rat IgG Antibody Peroxidase Conjugation, Goat Fab Anti-Rat IgG HRP Conjugated Antibody