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Catalog Number | orb348483 |
---|---|
Category | Antibodies |
Description | Fab Rabbit IgG (H&L) antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | DOT, ELISA, IHC, WB |
Reactivity | Rabbit |
Isotype | IgG Fab |
Immunogen | Rabbit IgG whole molecule |
Antibody Type | Secondary Antibody |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:4,000 - 1:20,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c). |
Conjugation | Unconjugated |
Storage | Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | Goat Fab Anti-Rabbit IgG Antibody, Goat Fab Fragme Read more... |
Note | For research use only |
Application notes | Fab Anti-Rabbit IgG (H&L) Antibody has been tested by dot blot and SDS-PAGE and is suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. |
Expiration Date | 12 months from date of receipt. |
SDS PAGE of Fab Anti-RABBIT IgG (GOAT) Antibody. Lane 1: 1x Reduced, Fab Anti-RABBIT IgG Antibody. Lane 2: Molecular Weight Ladder. Lane 3: 1x Non-Reduced, Fab Anti-RABBIT IgG Antibody. 4-20% Lonza SDS-PAGE; Coomassie Stained.
DOT, FC, FLISA, IF | |
Rabbit | |
Goat | |
Polyclonal | |
TRITC |
DOT, FLISA, IF, WB | |
Rabbit | |
Goat | |
Polyclonal | |
FITC |
ELISA, IHC, WB | |
Rabbit | |
Goat | |
Polyclonal | |
HRP |