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F(ab')2 Bovine IgG (H&L) Antibody Fluorescein Conjugated

Catalog Number: orb348075

DispatchUsually dispatched within 5-10 working days
$ 370.00
Catalog Numberorb348075
CategoryAntibodies
DescriptionF(ab')2 Bovine IgG (H&L) antibody (FITC)
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG F(ab')2
ConjugationFITC
ReactivityBovine
Form/AppearanceLyophilized
Concentration1.0 mg/mL
Buffer/Preservatives0.01% (w/v) Sodium Azide
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Bovine IgG and Bovine Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c). Limited reactivity will occur against Armenian Hamster IgG.
ImmunogenBovine IgG whole molecule
Tested applicationsDOT, FC, FLISA, IF
Dilution rangeFLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
Application notesF(ab')2 Anti-Bovine IgG Fluorescein conjugated antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Antibody TypeSecondary Antibody
StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Alternative namesRabbit F(ab')2 Anti-Bovine fluorescein Conjugated
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NoteFor research use only
F(ab')2 Bovine IgG (H&L) Antibody Fluorescein Conjugated

Dot Blot results of Rabbit F(ab')2 Anti-Bovine IgG Antibody Fluorescein Conjugated. Dots are Bovine IgG at (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Blocking: orb348637 for 30 min at RT. Primary Antibody: Rabbit F(ab')2 Anti-Bovine IgG Antibody FITC at 1 µg/ml for 1hr at RT. Secondary Antibody: none.