F4/80 antibody [C2C3], C-term

• Catalog Number: orb555999
• Category: Antibodies
• Description: Rabbit polyclonal antibody to F4/80
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human EMR1. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Dilution range: WB: 1:500-1:3000 , IHC-P: 1:100-1:1000
• Form/Appearance: Liquid: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Purity: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• MW: 98
• Target: F4/80
• UniProt ID: Q14246
• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
• Buffer/Preservatives: 0.025% ProClin 300. 1XPBS (pH7), 1% rAlbumin, 20% Glycerol
• Alternative names: adhesion G protein-coupled receptor E1 , EMR1 , TM
• Note: For research use only
• Application notes: Positive control: rat liver , C8D30 , NIH-3T3 , Raw264.7 , C2C12
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of PC13 xenograft using F4/80 antibody

Western blot analysis of Various whole cell using F4/80 antibody

Western blot analysis of rat liver lysate using F4/80 antibody

Western blot analysis of Jurkat whole cell lysate(Lane 1), Raji cells(Lane 2), K562 whole cell lysate(Lane 3) using F4/80 antibody

Western blot analysis of Raji cells using alpha Skeletal Muscle Actin antibody