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Catalog Number | orb1261309 |
---|---|
Category | Antibodies |
Description | EZH2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human EZH2 (NP_001190176.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 78-110kDa |
Target | EZH2 |
UniProt ID | Q15910 |
Storage | Store at -20˚°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | EZH2, WVS, ENX1, EZH1, KMT6, WVS2, ENX-1, EZH2b, K Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using EZH2 antibody (orb1261309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat testis using EZH2 antibody (orb1261309) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using EZH2 antibody (orb1261309) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using EZH2 antibody (orb1261309) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using EZH2 antibody (orb1261309) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using EZH2 antibody (orb1261309) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using EZH2 antibody (orb1261309) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using EZH2 antibody (orb1261309).
ELISA, IHC-P, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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