EWSR1 Antibody (C-term)

SKU: orb1935685

Description

EWSR1 Antibody (C-term)

Images & Validation

• Tested Applications: IF, WB
• Dilution range: WB - 1:1000, IF - 1:10-50
• Reactivity: Human, Mouse
• Predicted Reactivity: Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 68478 Da

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

EWS

EWSR1 Antibody (C-term) western blot analysis in Hela cell line lysates (35 ug/lane). This demonstrates the EWSR1 antibody detected the EWSR1 protein (arrow).

Fluorescent confocal image of Hela cell stained with EWSR1 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EWSR1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). EWSR1 immunoreactivity is localized to Nucleus significantly.

Quick Database Links

UniProt

Q01844