EWSR1 Antibody (C-term)

• Catalog Number: orb1926793
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB51591
• Tested applications: IF, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Dilution range: IF: 1:25, WB: 1:1000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 68478 Da
• Target: This EWSR1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 639-674 amino acids from the C-terminal region of human EWSR1.
• UniProt ID: Q01844
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: RNA-binding protein EWS, EWS oncogene, Ewing sarco
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-EWSR1 Antibody (C-term) at 1:1000 dilution. Lane 1: Hela whole cell lysates. Lane 2: K562 whole cell lysates. Lane 3: NIH/3T3 whole cell lysates. Lane 4: A549 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 68 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling EWSR1 at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing nuclear staining on Hela cell line. Cytoplasmic actin is detected with Alexa Fluor 555 conjugated with Phalloidin at 1/100 dilution (red).