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Catalog Number | orb385819 |
---|---|
Category | Antibodies |
Description | Estrogen receptor (ER) is an important regulator of growth and differentiation in the mammary gland. ER presence in breast tumors indicates an increased likelihood of response to anti-estrogen therapy. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | NR3Ga-3 |
Tested applications | FACS, IHC-P, WB |
Reactivity | Human |
Isotype | Mouse IgG1, kappa |
Immunogen | Recombinant human ER alpha protein (aa 2-185) was used as the immunogen. |
Dilution range | Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This ER antibody is available for research use only. |
Entrez | 2099 |
Storage | Store the ER antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | Variations in protocols, secondaries and substrates may require the ER antibody to be titered for optimal performance. |
Expiration Date | 12 months from date of receipt. |
Flow cytometric analysis of MCF-7 cells using ER antibody
Immunohistochemical staining of human Breast carcinoma using ER antibody
Western blot analysis of MCF-7 cell lysate using ER antibody
FACS, IHC-P, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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