Estrogen Receptor alpha Antibody

• Catalog Number: orb248339
• Category: Antibodies
• Description: Estrogen receptor (ER) is an important regulator of growth and differentiation in the mammary gland. ER presence in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. Clone ER506 antibody strongly stains nuclei of epithelial cells in breast carcinomas.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: ER506
• Tested applications: FACS, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant human ER alpha protein (aa 2-185) was used as the immunogen.
• Dilution range: Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml (1)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This ER antibody is available for research use only.
• Entrez: 2099
• Storage: Store the ER antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Variations in protocols, secondaries and substrates may require the ER antibody to be titered for optimal performance.1. FFPE testing requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and cooling at RT for 20 minutes.
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of MCF-7 cells using Estrogen R antibody

FACS staining of MCF-7 cells using Estrogen R antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded human breast invasive ductal carcinoma tissue using Estrogen R antibody

Western blot analysis of MCF-7 cell lysate using Estrogen R antibody