ER alpha Antibody / Estrogen Receptor alpha

SKU: orb1823106

Description

Estrogen receptor (ER) belongs to the steroid receptor superfamily of nuclear receptors. It is a protein with 553 amino acids. The receptor molecule has three domains, i.e. the central DNA-binding domain, the hormone-binding domain at the C-terminal, and the transcription-activating domain at the N-terminal. ER mediates regulatory functions of female sex steroids, mainly 17 (E2), on growth, differentiation and function in several target tissues, including female and male reproductive tract, mammary gland, and skeletal and cardiovascular systems. Studies have shown ER Alpha is present in the nuclei of epithelial cells in normal breast and endometrial tissues, as well as a subset of breast carcinomas. Studies with immunohistochemical assay show that positive steroid hormone status has predicted favorable overall, survival, independently of hormonal treatment. Secondly, ER Alpha can be used as a tumor marker, preferentially in combination with an antibody to progesterone receptor, e.g., in the classification of adenocarcinomas.

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the ER alpha antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: ESR1/7444R
• Immunogen: A recombinant partial protein sequence (within amino acids 495-595) from the human protein was used as the immunogen for the ER alpha antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Formalin fixed paraffin embedded human breast carcinoma tissue with ER alpha antibody (clone ESR1/7444R). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

Quick Database Links

UniProt

P03372