ER alpha Antibody / Estrogen Receptor alpha

SKU: orb1823051

Description

This mAb is specific to ER alpha and shows minimal cross-reaction with other members of the family. Epitope of this mAb is mapped between amino acids 495-595 of E/F domain. Estrogen Receptor is an important regulator of growth and differentiation in the mammary gland. Presence of Estrogen Receptor in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.

Images & Validation

• Tested Applications: FACS, IF
• Dilution Range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the ER alpha antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: AER311
• Immunogen: Estrogen Receptor purified from cow uterus was used as the immunogen for the ER alpha antibody. The epitope of this mAb is mapped between amino acids 495-595.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Analysis of a HuProt (TM) microarray containing more than 19000 full-length human proteins using ER alpha antibody (clone AER311). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.

Quick Database Links

UniProt

P03372