TFF1 Antibody

SKU: orb1252701

Description

TFF1 Antibody

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Reactivity: Human, Primate
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlIHC (FFPE): 0.5-1.0 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of FFPE tissues REQUIRES digestion with Pronase E at 1mg/ml in PBS for 15 min at 37°C. Heat Induced Epitope Retrieval is not suitable for this antibody.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: GE2
• Immunogen: Amino acids CFDDTVRGVPWCFYPNTIDVPPEEECEF (57-84) from the C-terminus of human Estrogen inducible protein pS2 was used as the immunogen for this antibody.
• Target: TFF1
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Disclaimer: For research use only

Alternative Names

Trefoil factor 1, Breast cancer estrogen-inducible protein, PNR-2, Polypeptide P1.A, hP1.A, Protein pS2, TFF1, BCEI, PS2

IHC staining of human breast cancer with Estrogen Inducible Protein pS2 antibody.

IHC testing of FFPE human ovarian carcinoma with Estrogen Inducible Protein pS2 antibody.

SDS-PAGE Analysis of Purified, BSA-Free Estrogen Inducible Protein pS2 Antibody (clone GE2). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

Gene Symbol

TFF1

UniProt

P04155