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| Catalog Number | orb1252701 |
|---|---|
| Category | Antibodies |
| Description | Estrogen Inducible Protein pS2 Antibody [GE2] |
| Target | TFF1 |
| Clonality | Monoclonal |
| Isotype | IgG1, kappa |
| Conjugation | Unconjugated |
| Reactivity | Human, Primate |
| Form/Appearance | Liquid |
| Concentration | 0.2 mg/mL |
| Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
| Purification | Protein G affinity chromatography |
| Immunogen | Amino acids CFDDTVRGVPWCFYPNTIDVPPEEECEF (57-84) from the C-terminus of human Estrogen inducible protein pS2 was used as the immunogen for this antibody. |
| UniProt ID | P04155 |
| Tested applications | FC, IF, IHC, WB |
| Application notes | Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlIHC (FFPE): 0.5-1.0 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of FFPE tissues REQUIRES digestion with Pronase E at 1mg/ml in PBS for 15 min at 37°C. Heat Induced Epitope Retrieval is not suitable for this antibody.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
| Antibody Type | Primary Antibody |
| Clone Number | GE2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Trefoil factor 1, Breast cancer estrogen-inducible Read more... |
| Research Area | Signal Transduction |
| Note | For research use only |
![Estrogen Inducible Protein pS2 Antibody [GE2]](/images/pub/media/catalog/product/NewWebsite/15/orb1252701_1.jpg)
IHC staining of human breast cancer with Estrogen Inducible Protein pS2 antibody.
![Estrogen Inducible Protein pS2 Antibody [GE2]](/images/pub/media/catalog/product/NewWebsite/15/orb1252701_2.jpg)
IHC testing of FFPE human ovarian carcinoma with Estrogen Inducible Protein pS2 antibody.
![Estrogen Inducible Protein pS2 Antibody [GE2]](/images/pub/media/catalog/product/NewWebsite/15/orb1252701_3.jpg)
SDS-PAGE Analysis of Purified, BSA-Free Estrogen Inducible Protein pS2 Antibody (clone GE2). Confirmation of Integrity and Purity of the Antibody.
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