TFF1 Antibody

• Catalog Number: orb1258503
• Category: Antibodies
• Description: TFF1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-84 of human TFF1 (NP_003216.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:1000IHC: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 12kDa
• Target: TFF1
• UniProt ID: P04155
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: Trefoil factor 1, Breast cancer estrogen-inducible
• Note: For research use only
• Application notes: WB: 1:500 - 1:1000IHC: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of BT-474 cells, using TFF1 antibody (orb1258503) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human breast cancer using TFF1 antibody (orb1258503) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using TFF1 antibody (orb1258503) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat lung using TFF1 antibody (orb1258503) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using TFF1 antibody (orb1258503) at dilution of 1:200 (40x lens).