ESRRA Antibody

SKU: orb1262593

Description

ESRRA Antibody

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Immunogen: This ESRRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 131-159 amino acids from the Central region of human ESRRA.
• Target: ESRRA
• Molecular Weight: 46 kDa
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

Steroid hormone receptor ERR1, Estrogen receptor-like 1, Estrogen-related receptor alpha, ERR-alpha, Nuclear receptor subfamily 3 group B member 1, ESRRA, ERR1, ESRL1, NR3B1

Western blot analysis of ESRRA Antibody in A375 cell line lysates (35 ug/lane)

Formalin-fixed and paraffin-embedded human kidney carcinoma reacted with ESRRA Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometric analysis of 293 cells using ESRRA Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of ESRRA using rabbit polyclonal ESRRA Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ESRRA gene.

Confocal immunofluorescent analysis of ESRRA Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Quick Database Links

Gene Symbol

ESRRA

UniProt

P11474

RefSeq:P11474