MAPK1 Antibody

SKU: orb750464

Description

ERK1/2 antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution Range: ELISA: 1:2,000 - 1:10,000, IP: 5.0 µg/mg sample, WB: 1:1,000-1:,2000
• Reactivity: Human, Mouse
• Application Notes:
  This antiserum was tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 42 kDa in size corresponding to p42 MAP Kinase (ERK2) by western blotting in the appropriate cell lysate or extract. p42 MAP Kinase (ERK2) is a ubiquitous protein kinase target for Ras and Raf. The following cell lines have been assayed by immunoblot and were found to be positive for p42 MAP Kinase (ERK2) using this reagent: U937, HeLa, NIH-3T3, RAW 264.7, LNCaP and HEK whole cell lysates.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Antiserum
• Immunogen: Anti-Erk2 antibody was prepared by repeated immunizations with an Erk2 containing fusion protein. The epitope maps near the carboxy-terminus of human p42 MAP Kinase (ERK2) protein. The epitope is identical to the corresponding sequence in mouse and differs from the rat sequence by a single, conservative amino acid substitution.
• Target: MAPK1
• Purity: This antiserum is directed against human p42 MAP Kinase (ERK2) protein and is useful in determining its presence by immunoblotting. No reactivity is observed against p44 MAP Kinase (ERK1). Cross reactivity is expected with p44 MAP Kinase (ERK1) proteins from human and mouse sources. Reactivity to rat tissues is also anticipated due to high sequence homology. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 85 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Erk2 Antibody, MAP kinase 1, MAP kinase 2, p42-MAPK

Western blot using Biorbyt's Affinity Purified anti-p42 MAP Kinase (ERK2) antibody shows detection of ERK2 in several whole cell lysates. Lane 1: HeLa (p/n orb348668), Lane 2: A431 (p/n orb348665), and Lane 3: NIH3T3 (p/n orb348714). Primary antibody: Anti-p42 at 1:1000. Secondary antibody: HRP Goat-a-Rabbit 1:4000 dilution with visualization via ECL. Film exposure was approximately 1'. Other detection systems will yield similar results.

Quick Database Links

Gene Symbol

MAPK1

UniProt

P28482

RefSeq:66932916