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Catalog Number | orb626803 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to ERK1/2 |
Target | ERK1/2 |
Clonality | Monoclonal |
Species/Host | Mouse |
Isotype | IgG1 |
Conjugation | Unconjugated |
Reactivity | Human, Mouse |
Form/Appearance | liquid |
Purity | ≥95% as determined by SDS-PAGE |
Purification | Protein A+G purified |
Immunogen | mitogen-activated protein kinase 3 |
UniProt ID | P27361 |
MW | 38-44 kDa |
Tested applications | ELISA, IF, IHC, WB |
Dilution range | WB:1:500-1:5000; IHC: 1:100-1:400; IF: 1:20-1:200 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | ERK, ERK 1, ERK1, ERK1/2, ERT2, HS44KDAP, HUMKER1A Read more... |
Note | For research use only |
Immunofluorescent analysis of HepG2 cells using orb626803 (ERK1/2 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
HeLa cells were subjected to SDS PAGE followed by western blot with orb626803 (ERK1/2 Antibody) at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using orb626803 (ERK1/2 Antibody) at dilution of 1:200. Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.
FC, ICC, IF, IHC-Fr, IHC-P | |
Bovine, Canine, Equine, Gallus, Guinea pig, Porcine, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IF, IHC | |
Bovine, Canine, Equine, Gallus, Guinea pig, Porcine, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC-Fr, IHC-P, WB | |
Zebrafish | |
Human, Mouse, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |
ICC, IF, IHC-Fr, IHC-P, WB | |
Bovine, Canine, Equine, Gallus, Goat, Porcine, Rabbit, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC-Fr, IHC-P, WB | |
Mouse, Rat | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |