ERK1/2 Mouse Monoclonal Antibody

SKU: orb626803

Description

Mouse monoclonal antibody to ERK1/2

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: WB:1:500-1:5000; IHC: 1:100-1:400; IF: 1:20-1:200
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Immunogen: mitogen-activated protein kinase 3
• Target: ERK1/2
• Molecular Weight: 38-44 kDa
• Purity: ≥95% as determined by SDS-PAGE
• Purification: Protein A+G purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: liquid
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ERK, ERK 1, ERK1, ERK1/2, ERT2, HS44KDAP, HUMKER1A, Insulin stimulated MAP2 kinase, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, p44 ERK1, p44 MAPK, P44ERK1, P44MAPK, PRKM3

Immunofluorescent analysis of HepG2 cells using orb626803 (ERK1/2 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

HeLa cells were subjected to SDS PAGE followed by western blot with orb626803 (ERK1/2 Antibody) at dilution of 1:1000.

Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using orb626803 (ERK1/2 Antibody) at dilution of 1:200. Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

Quick Database Links

Gene Symbol

ERK1/2

UniProt

P27361