ERK1 N-Term Antibody

SKU: orb420329

Description

ERK1/2 antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:40,000, WB: 1.0 ug/mL
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Anti-ERK 1 (RABBIT) antibody has been tested in ELISA and Western Blotting. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 43 kDa.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-ERK1 Antibody was produced in rabbits by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus conjugated to KLH.
• Purity: This affinity purified antibody is directed against human ERK1 protein. Anti-ERK1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using synthetic peptide coupled to agarose beads followed by cross adsorption to remove any unwanted reactivity. Cross reactivity is expected to occur with human, mouse and rat based on sequence identity of the peptide immunogen. This antibody does not react with the ERK2 isoform.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-ERK-1 Antibody, MAPK3, ERK 1, ERK1, PRKM3, Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, microtubule-associated protein 2 kinase, p44-ERK1

Western Blot of Rabbit Anti-ERK1 N-Term Antibody. Lane 1: Molecular weight marker. Lane 2: ERK1 double mutant WC Lysate. Lane 3: ERK2 protein. Lane 4: Mouse Brain WC Lysate (p/n orb348715). Lane 5: HEK293 WC Lysate (p/n orb348669). Load: 10 ng lysate or 50 ng protein. Blocking: Universal Blocking Buffer (p/n orb348644) for 30 min RT. Primary antibody: Anti-ERK1 N-Term at 1 µg/ml overnight at 4°C. Secondary antibody: Goat anti-Rabbit HRP (p/n orb347654) 1:40000 in buffer (p/n orb348644) for 30 min at RT. Expect: ~43kDa.

Quick Database Links

UniProt

P27361