ERBB3 Antibody

• Catalog Number: orb1247982
• Category: Antibodies
• Description: ERBB3 Antibody
• Target: ERBB3
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-HSRLFPKANAQRT
• UniProt ID: P21860
• Tested applications: ELISA, FC, IF, IHC
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Immunohistochemistry:Paraffin embedded Human Lung. Recommend concentration: 5ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes of MCF7 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ERBB3, HER3, v-erb-b2 erythroblastic leukemia vira
• Research Area: Signal Transduction
• Note: For research use only
• NCBI: NP_001973.2
• Expiration Date: 12 months from date of receipt.

orb1247982 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing plasma membrane/cytoplasmic staining.

orb1247982 (5 ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247982 Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).