ERBB3/HER3 Antibody

• Catalog Number: orb19129
• Category: Antibodies
• Description: Goat polyclonal antibody to ERBB3
• Target: ERBB3 / HER3
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: HSRLFPKANAQRT
• RRID: AB_10755551
• MW: 148
• Tested applications: ELISA, FC, IF, IHC
• Dilution range: ELISA: 1:32000, IHC-P: 5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 150kDa band observed in human lysates of T cell line JURKAT (calculated MW of 148kDa according to NP_001973.2). Recommended concentration: 0.1-0.3 μg/ml. A minor band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti ERBB3 antibody, anti HER3 antibody, anti v-er
• Research Area: Cancer, Epigenetics
• Note: For research use only
• Entrez: 2065
• Expiration Date: 12 months from date of receipt.

orb19129 (0.1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

orb19129 (5µg/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunofluorescence analysis of HeLa cells using ERBB3 antibody

Flow cytometric analysis of MCF7 cells using ERBB3 antibody