ERBB3/HER3 Antibody

SKU: orb19129

Description

Goat polyclonal antibody to ERBB3

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC
• Dilution range: ELISA: 1:32000, IHC-P: 5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 150kDa band observed in human lysates of T cell line JURKAT (calculated MW of 148kDa according to NP_001973.2). Recommended concentration: 0.1-0.3 μg/ml. A minor band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: ERBB3 / HER3
• Molecular Weight: 148
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti ERBB3 antibody, anti HER3 antibody, anti v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) antibody, anti HGNC:3431 antibody, anti ErbB-3 antibody, anti MDA-BF-1 antibody, anti c-erbB-3 antibody, anti c-erbB3 antibody, anti erbB3-S antibody, anti p180-ErbB3 antibody, anti p45-sErbB3 antibody, anti p85-sErbB3 antibody, anti erbB-3 antibody, anti LCCS2 antibody, anti MGC88033 antibody, anti receptor tyrosine-protein kinase erbB-3 antibody, anti v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3 antibody

orb19129 (0.1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

orb19129 (5µg/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunofluorescence analysis of HeLa cells using ERBB3 antibody

Flow cytometric analysis of MCF7 cells using ERBB3 antibody

Quick Database Links

Gene Symbol

ERBB3 / HER3

NCBI Gene ID

2065