ERAS Antibody

SKU: orb1268114

Description

ERAS Antibody

Research Area

Cancer Biology, Cell Biology, Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Application Notes:
  For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Immunogen: This ERAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human ERAS.
• Target: ERAS
• Molecular Weight: 25 kDa
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GTPase ERas, E-Ras, Embryonic stem cell-expressed Ras, ERAS, HRAS2, HRASP

Western blot analysis of ERAS Antibody in K562 cell line lysates (35 ug/lane)

Formalin-fixed and paraffin-embedded human brain tissue reacted with ERAS antibody (A28), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Immunofluorescence analysis of anti-ERAS Antibody in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H + L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.

Flow cytometric analysis of hela cells using ERAS Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Quick Database Links

Gene Symbol

ERAS

UniProt

Q7Z444

RefSeq:Q7Z444