ERAS Antibody (N-term)

• Catalog Number: orb1935563
• Category: Antibodies
• Description: ERAS Antibody (N-term)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: Q7Z444
• MW: 25287 Da
• Tested applications: FC, IF, IHC-P, WB
• Dilution range: WB - 1:1000, IHC-P - 1:100-500, IF - 1:10-50, FC - 1:10-50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: HRAS2, HRASP
• Research Area: Cancer Biology, Signal Transduction, Stem Cell & D
• Note: For research use only

Western blot analysis of ERAS Antibody (N-term) in K562 cell line lysates (35 ug/lane). ERAS (arrow) was detected using the purified Pab.

Flow cytometric analysis of hela cells using ERAS Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunofluorescence analysis of anti-ERAS Antibody (N-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.

Formalin-fixed and paraffin-embedded human brain tissue reacted with ERAS antibody (N-term) (A28), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.