ERAS Antibody (N-term) (F66)

• Catalog Number: orb1935562
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This ERAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-81 amino acids from the N-terminal region of human ERAS.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: Q7Z444
• MW: 25287 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: IF: 1:100, WB: 1:1000, IHC-P: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB13001
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: GTPase ERas, E-Ras, Embryonic stem cell-expressed
• Research Area: Cancer Biology, Signal Transduction, Stem Cell & D
• Note: For research use only
• NCBI: NP_853510.1

Western blot analysis of ERAS Antibody (N-term) (F66) in HL60 cell line lysates (35 ug/lane). ERAS (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue reacted with ERAS antibody (N-term) (F66), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Fluorescent confocal image of SY5Y cells stained with ERAS (N-term) (F66) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ERAS (N-term) (F66) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1 h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ERAS immunoreactivity is localized to the plasma membrane of SY5Y cells.