(-)-Epigallocatechin Gallate

SKU: orb1304766

Description

(-)-Epigallocatechin Gallate (EGCG) is a bioactive tea polyphenol that acts as a potent antioxidant and enzyme inhibitor. It is widely used in research to study mechanisms in oncology, epigenetics, and oxidative stress, with applications in both cell-based and animal models.

Research Area

Cell Biology, Immunology & Inflammation, Infectious Disease & Virology, Metabolism Research, Protein Biochemistry, Signal Transduction

Key Properties

• CAS Number: 989-51-5
• MW: 458.37
• Purity: 99.99% (May vary between batches)
• Formula: C₂₂H₁₈O₁₁
• SMILES: O(C(=O)C1=CC(O)=C(O)C(O)=C1)[C@H]2[C@H](OC=3C(C2)=C(O)C=C(O)C3)C4=CC(O)=C(O)C(O)=C4
• Target: Autophagy,Mitochondrial Metabolism,Reactive Oxygen Species,HIV Protease,Ferroptosis,Apoptosis,Endogenous Metabolite
• Solubility: DMSO:242.5 mg/mL (529.05 mM);Ethanol:45.8 mg/mL (99.92 mM);H2O:50 mg/mL (109.08 mM)

Bioactivity

• Target IC50:
  Jurkat cells:1 μM|COLO 201 cells:38 μM|BxPC-3 cells:33 μM|L929 cells:82.3 μM|SW48 cells:195 μM|HT22 cells:23.8 μM|NCI-H1299 cells:20.6 μM|A549 cells:29 μM|CHO cells:3.2 μM|HeLa cells:1.08 μM|3T3-L1 cells:112 μM|SK-MEL-2 cells:31 μM|HSCT6 cells:9.9 μM|HCT116 cells:5 μM|MDCK cells:41.25 μM
• In Vivo:
  METHODS: To investigate the protective effect against acute injury, (-)-Epigallocatechin Gallate (200 mg/kg) was administered to ICR mice by gavage once a day for one week, followed by intraperitoneal injection of LPS (15 mg/kg) to induce acute injury. RESULTS: (-)-Epigallocatechin Gallate protects mice from LPS-induced acute stress, while stabilizing gut microorganisms, improving sphingolipid metabolism, and inhibiting harmful metabolite levels.
• In Vitro:
  METHODS: Human thyroid cancer cells TT, TPC-1 and ARO were treated with (-)-Epigallocatechin Gallate (10-200 µM) for 24 h. Cell viability was detected using MTS assay.
• Cell Research:
  LoVo, SW480, HCT-8, and HT-29 cells are seeded in 96-well plates at a concentration of 5×103 cells; each cell line is totally seeded in the 12 wells. Complete medium is added to the wells, up to 200 μL; the medium contains 0 μg/mL, 10 μg/mL, 20 μg/mL, and 35 μg/mL of epigallocatechol gallate. The inhibition rate=[1 - (absorbance of Epigallocatechol Gallate group - absorbance of control group)/(absorbance of control group - absorbance of blank control group)] × 100.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

cell proliferation., colorectal cancer, Endogenous Metabolite, ()Epigallocatechin Gallate, ( ) Epigallocatechin Gallate, Autophagy, inhibit, LPS-induced bone resorption, Mitochondrial Metabolism, MitochondrialMetabolism, Inhibitor, HIV, HIV Protease, HIVProtease, EGCG, EndogenousMetabolite, Epigallocatechin Gallate, Epigallocatechol Gallate, Ferroptosis, FB-2 cells, Thyroid cancer, ReactiveOxygenSpecies, Reactive Oxygen Species

Compound Identifiers

PubChem CID

65064