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EPHA5 Antibody
Description
Research Area
Images & Validation
−| Tested Applications | IHC-P, WB |
|---|---|
| Dilution Range | WB - 1:2000, IHC-P - 1:100-500 |
| Reactivity | Human |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1κ |
| Molecular Weight | 114803 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Formalin-fixed and paraffin-embedded human brain tissue reacted with EPHA5 Monoclonal Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of anti-EPHA5 Monoclonal Antibody by EPHA5 recombinant protein (Fragment). EPHA5 (Fragment) protein (arrow) was detected using the purified Mab. (1:2000)

Western blot analysis of lysate from U-251 MG cell line, using EphA5 Antibody. diluted at 1:1000. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary Antibody. Lysate at 35 μg.
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Protocol Information
EPHA5 Antibody (orb1939226)
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