EPHA5 Antibody

SKU: orb1939226

Description

Purified Mouse Monoclonal Antibody (Mab)

Research Area

Neuroscience

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:10-50
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1κ
• Clone No.: 46CT61.6.4
• Immunogen: Purified His-tagged EPHA5 protein(Fragment) was used to produced this monoclonal antibody.
• Target: EPHA5
• Molecular Weight: 114803 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Ephrin type-A receptor 5, Brain-specific kinase, EPH homology kinase 1, EHK-1, EPH-like kinase 7, EK7, hEK7, EPHA5, BSK, EHK1, HEK7, TYRO4

Formalin-fixed and paraffin-embedded human brain tissue reacted with EPHA5 Monoclonal Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of anti-EPHA5 Monoclonal Antibody by EPHA5 recombinant protein (Fragment). EPHA5 (Fragment) protein (arrow) was detected using the purified Mab. (1:2000)

Western blot analysis of lysate from U-251 MG cell line, using EphA5 Antibody. diluted at 1:1000. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary Antibody. Lysate at 35 μg.

Quick Database Links

Gene Symbol

EPHA5

UniProt

P54756

RefSeq (Protein):NP_004430.4, NP_872272.2