Phospho-EPH A3+A4+A5 (Tyr779) Rabbit Polyclonal Antibody

SKU: orb100971

Description

Phospho-EPH A3+A4+A5 (Tyr779) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human around the phosphorylation site of EPH 3 Tyr779 AA(p-Y)TT
• Target: EPHA3
• Molecular Weight: 108 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

EPH A3+A4+A5(phospho Y779+Y779+Y833); EPH A3+A4+A5(phospho Y779); p-EPH A3+A4+A5(phospho Y779); CEK7; EHK 1; EHK1; EK4; EK7; EK8; EPH homology kinase 1; EPH like kinase 4; EPH like kinase 7; EPH like kinase 8; Eph like tyrosine kinase 1; EPH receptor A3; EPH receptor A4; EPH receptor A5; Ephrin type A receptor 3; Ephrin type A receptor 4; Ephrin type A receptor 5; ETK; ETK1; HEK; HEK4; HEK7; HEK8; Human embryo kinase 1; Receptor protein tyrosine kinase HEK7; Receptor protein tyrosine kinase HEK8; SEK; TYRO1; TYRO1 protein tyrosine kinase; TYRO4; TYRO4 protein tyrosine kinase; Tyrosine protein kinase receptor EHK 1; Tyrosine protein kinase receptor ETK1; Tyrosine protein kinase receptor SEK; Tyrosine protein kinase TYRO1; EPHA3_HUMAN; EPHA4_HUMAN; EPHA5_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-EPH A3+A4+A5 (Tyr779)) Polyclonal Antibody, Unconjugated (orb100971) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-EPH A3+A4+A5 (Tyr779)) Polyclonal Antibody, Unconjugated (orb100971) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human HeLa cell lysates, Lane 2: Human HeLa (Serum-starve cells overnight, treat with the freshly made Pervanadate for 20min at 37°C), Primary: Anti-phospho-EPH A3+A4+A5 (Tyr779) (orb100971) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 108 kDa, Observed band size: 105 kDa.

Quick Database Links

Gene Symbol

EPHA3

UniProt

P29320