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Description
Research Area
Neuroscience
Images & Validation
−Item 1 of 6
| Tested Applications | IHC-P, WB |
|---|---|
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit Ig |
| Immunogen | This EphA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 875-904 amino acids from the C-terminal region of human EphA4. |
| Target | EPHA4 |
| Molecular Weight | 110 kDa |
| Purification | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Ephrin type-A receptor 4, EPH-like kinase 8, EK8, hEK8, Tyrosine-protein kinase TYRO1, Tyrosine-protein kinase receptor SEK, EPHA4, HEK8, SEK, TYRO1
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Western blot analysis of lysates from Hela, HUVEC cell line (from left to right), using EPHA4 Antibody at 1:1000 at each lane.
Western blot analysis of lysates from Hela, NCI-H460, HUVEC cell line (from left to right), using EPHA4 Antibody at 1:1000 at each lane.
Western blot analysis of lysates from Hela, NCI-H460, mouse NIH/3T3 cell line and human ovary tissue lysate (from left to right), using EPHA4 Antibody at 1:1000 at each lane.
Western blot analysis of anti-EphA4 Pab in NCI-H460 cell lysate
Western blot analysis of EPHA4 using EphA4 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA4 gene (Lane 2).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
EPHA4 Antibody (orb1271019)
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