EpCAM Rabbit Polyclonal Antibody

SKU: orb323292

Description

EpCAM Rabbit Polyclonal Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Dilution Range: WB: 1:100-300, IHC-P: 1:100-500 (based on 0.5 mg/ml)
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human EpCAM. Please contact us for the exact immunogen sequence. The peptide is available as orb374948.
• Target: EpCAM. Recognizes endogenous levels of EpCAM protein
• Molecular Weight: 35 kDa
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

WB analysis of rat liver (lane 1), human endometrial cancer (lane 2), human breast cancer (lane 3), hela cells (lane 4) using EpCAM antibody (2.5 ug/ml)

Western blot analysis of rat liver (lane 1), human endometrial cancer (lane 2), human breast cancer (lane 3), hela cells (lane 4) using EpCAM antibody (2.5 ug/ml)

IHC-P staining of human endometrial cancer tissue using EpCAM antibody (2.5 ug/ml)

Immunohistochemical staining of human endometrial cancer tissue using anti-EpCAM (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded rat liver tissue using anti-EpCAM (2.5 ug/ml)

Quick Database Links

Gene Symbol

EpCAM. Recognizes endogenous levels of EpCAM protein

UniProt

O55159, P16422, Q99JW5

NCBI Gene ID

4072