EpCAM Antibody

• Catalog Number: orb317544
• Category: Antibodies
• Description: EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Epcam/1159
• Tested applications: IHC-P
• Reactivity: Mouse, Rat
• Isotype: Mouse IgG1
• Immunogen: Recombinant full-length rat protein was used as the immunogen for the rat EpCAM antibody.
• Dilution range: Immunohistochemistry (FFPE): 1:50-1:100 for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (3)
• Purity: Unpurified high titer supernatant
• Conjugation: Other
• Formula: Bioreactor concentrate with 0.05% sodium azide
• Hazard Information: This rat EpCAM antibody is available for research use only.
• UniProt ID: O55159
• Storage: Store the rat EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: Bioreactor concentrate with 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the rat EpCAM antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.; 2. The epitopes of mAbs Epcam/1158 and Epcam/1159 monoclonal antibodies are different.3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Rat lung using rat EpCAM antibody

Immunohistochemical staining of Rat colon using rat EpCAM antibody