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Catalog Number | orb317543 |
---|---|
Category | Antibodies |
Description | EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | Epcam/1158 |
Tested applications | IHC-P |
Reactivity | Mouse, Rat |
Isotype | Mouse IgG1, kappa |
Immunogen | Recombinant full-length rat protein was used as the immunogen for the rat EpCAM antibody. |
Dilution range | Immunohistochemistry (FFPE): 1:50-1:100 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (3) |
Purity | Unpurified high titer supernatant |
Conjugation | Other |
Formula | Bioreactor concentrate with 0.05% sodium azide |
Hazard Information | This rat EpCAM antibody is available for research use only. |
UniProt ID | O55159 |
Storage | Store the rat EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | Bioreactor concentrate with 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the rat EpCAM antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. 2. The epitopes of mAbs Epcam/1158 and Epcam/1159 monoclonal antibodies are different.3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical staining of Rat colon using rat EpCAM antibody
IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IF, IHC-P, WB | |
Canine, Feline, Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC, WB | |
Human, Mouse, Rabbit | |
Rabbit | |
Polyclonal | |
Unconjugated |
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