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Description
Research Area
Immunology & Inflammation
Images & Validation
−
| Tested Applications | FC, IF, IHC, WB |
|---|---|
| Dilution Range | Flow Cytometry (5-10ul/million cells); Immunofluorescence (1:50-1:100); Western Blot (1:100-1:200); Immunoprecipitation (5-10ul/500ug protein lysate);Immunohistochemistry (Frozen & formalin-fixed) (1:100-1:200 for 30 min at RT)(Formalin/paraffin tissues REQUIRE digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37C)Optimal dilution for a specific application should be determined. |
| Reactivity | Rat |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | SPM534 |
| Immunogen | Rat small intestine cells |
| Target | EPCAM |
| Molecular Weight | 40-43kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Bioreactor Concentrate with 0.05% Azide. Contact us if you require it in a different format. |
| Concentration | Bioreactor Concentrate |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TACSTD1; TROP1; Tumor-associated Calcium Signal Transducer 1, ECS-1, Epidermal Surface Antigen 1, ESA1, FLOT2, Flotillin-2, M17S1 (Membrane Component, Chromosome 17, Surface Marker-1), REG-1, Reggie-1, Reggie-2
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence























































