Recombinant EpCAM Antibody / Extracellular domain

SKU: orb2640565

Description

EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution range: Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Reactivity: Canine, Feline, Human
• Application Notes:
  Optimal dilution of the recombinant EpCAM antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: Mouse IgG1, kappa
• Clone No.: rEGP40/1372
• Immunogen: A portion of amino acids 100-224 (extracellular domain) was used as the immunogen for the recombinant EpCAM antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC testing of FFPE human skin with recombinant EpCAM antibody (clone rEPG40/1372). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human rectal mass tissue with recombinant EpCAM antibody (clone rEPG40/1372). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant EpCAM antibody (clone rEPG40/1372) as confirmation of integrity and purity.

Western blot testing of human MCF-7 cell lysate with recombinant EpCAM antibody (clone rEPG40/1372). Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).

IHC staining of FFPE cat small intestine with recombinant EpCAM antibody (clone rEGP40/1372). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using recombinant EpCAM antibody (clone rEGP40/1372). These results demonstrate the foremost specificity of the rEGP40/1372 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Quick Database Links

UniProt

P16422