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Catalog Number | orb389162 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to EpCAM |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | EGP40/837 |
Tested applications | FC, IF, IHC, WB |
Reactivity | Human |
Isotype | IgG1 |
Immunogen | Recombinant human TACSTD1 protein |
Concentration | Purified Ab with BSA and Azide at 200ug/ml |
Dilution range | FACS: 0.5-1 μg/million cells, IF/ICC: 1-2 μg/ml, WB: 0.5-1 μg/ml, IHC-P: 0.5-1 μg/ml |
Form/Appearance | 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
Conjugation | Unconjugated |
MW | 40-43kDa |
Target | EPCAM |
Entrez | 4072 |
UniProt ID | P16422 |
Expression System | HEK293 cells |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
Alternative names | anti Adenocarcinoma associated antigen antibody, a Read more... |
Note | For research use only |
Application notes | Ep-CAM / CD326 (Epithelial Marker) |
Expiration Date | 12 months from date of receipt. |
Flow cytometric analysis of MCF-7 Cells using EpCAM antibody
Immunofluorescence analysis of SK-OV-3 using EpCAM antibody
Immunofluorescence analysis of SK-OV-3 using EpCAM antibody
Immunohistochemical staining of human Colon carcinoma tissue using EpCAM antibody
Immunohistochemical staining of human Ovarian Carcinoma tissue using EpCAM antibody
Immunohistochemical staining of human Endometrial Carcinoma tissue using EpCAM antibody
Western blot analysis of HCT116 Cell Lysate using EpCAM antibody
FC, IF, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, IF, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FC, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, IF, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FC, IF, IHC, WB | |
Canine, Feline, Human | |
Mouse | |
Monoclonal | |
Unconjugated |