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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, ICC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human eNOS. |
| Target | NOS3 |
| Molecular Weight | Calculated MW: 133 kDa; Observed MW: 140 kDa |
| Purification | Affinity Chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Western blot analysis of eNOS in HepG2 lysates using eNOS antibody.

Western blot analysis of eNOS in HepG2 lysates using NOS3 antibody.

Immunohistochemistry analysis of paraffin-embedded Human brain using eNOS antibody. High-pressure and temperature Tris-EDTA pH 8.0 was used for antigen retrieval.Sample with blocking peptide on the right.

Western blot analysis of eNOS in Jurkat lysates treated with Insulin using eNOS antibody. The lane on the right is blocked with the synthesized peptide.
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Protocol Information
eNOS Rabbit pAb (orb1562986)
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