eNos Antibody (S1177)

• Catalog Number: orb1937922
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB29786
• Tested applications: FC, IF, WB
• Predicted Reactivity: Mouse, Porcine, Rat, Sheep
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:10~50, WB: 1:1000, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 133275 Da
• Target: This eNos antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1156-1183 amino acids from human eNos.
• UniProt ID: P29474
• NCBI: NP_001153582.1, NP_000594.2, NP_001153583.1, NP_001153581.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Nitric oxide synthase, endothelial, Constitutive N
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Confocal immunofluorescent analysis of eNos Antibody (S1177) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

eNos Antibody (S1177) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

eNos Antibody (S1177) western blot analysis in K562 cell line lysates (35 ug/lane). This demonstrates the eNos antibody detected the eNos protein (arrow).