eNos Antibody (S1177)

SKU: orb1937922

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Immunology & Inflammation, Metabolism Research, Neuroscience

Images & Validation

• Tested Applications: FC, IF, WB
• Dilution Range: IF - 1:10-50, WB - 1:1000, FC - 1:10-50
• Reactivity: Human
• Predicted Reactivity: Mouse, Porcine, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD41908
• Immunogen: This eNos antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1156-1183 amino acids from human eNos. Antigen Region: 1156-1183 aa.
• Target: NOS3 (HGNC:7876)
• Molecular Weight: 133275 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Nitric oxide synthase, endothelial, Constitutive NOS, cNOS, EC-NOS, Endothelial NOS, eNOS, NOS type III, NOSIII, NOS3

Confocal immunofluorescent analysis of eNos Antibody (S1177) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

eNos Antibody (S1177) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

eNos Antibody (S1177) western blot analysis in K562 cell line lysates (35 ug/lane). This demonstrates the eNos antibody detected the eNos protein (arrow).

Quick Database Links

Gene Symbol

NOS3 (HGNC:7876)

UniProt

P29474

RefSeq (Protein):NP_001153583.1, NP_001153581.1, NP_001153582.1, NP_000594.2