ENOB Antibody (Center)

• Catalog Number: orb1931365
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB19997
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Dilution range: WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 46987 Da
• Target: This ENOB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237-264 amino acids from the Central region of human ENOB.
• UniProt ID: P13929
• NCBI: NP_443739.3, NP_001180432.1, NP_001967.3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Beta-enolase, 2-phospho-D-glycerate hydro-lyase, E
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ENOB Antibody (Center) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ENOB Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

ENOB Antibody (Center) western blot analysis in RD cell line and mouse skeletal muscle, rat skeletal muscle lysates (35 ug/lane). This demonstrates the ENOB antibody detected the ENOB protein (arrow).