ENOB Antibody (Center)

• Catalog Number: orb1788251
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: 237-264 aa
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 46987 Da
• Target: This ENOB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237-264 amino acids from the Central region of human ENOB.
• UniProt ID: P13929
• NCBI: NP_001967.3, NP_001180432.1, NP_443739.3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: ENO3; Beta-enolase; 2-phospho-D-glycerate hydro-ly
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ENOB Antibody (Center) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of lysates from RD cell line, mouse skeletal muscle, rat skeletal muscle tissue lysate (from left to right), using ENOB Antibody (Center). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ENOB Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.