ENO1 antibody [N3C3]

• Catalog Number: orb555988
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ENO1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ICC, IHC, IHC-P, WB
• Reactivity: Human, Mouse, Rat, Zebrafish
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ENO1. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Dilution range: WB: 1:500-1:20000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000
• Form/Appearance: Liquid: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Purity: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• MW: 47
• Target: ENO1
• UniProt ID: P06733
• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
• Buffer/Preservatives: 0.01% Thimerosal. 0.1M Tris-Glycine (pH7), 10% Glycerol
• Alternative names: enolase 1 , ENO1L1 , HEL-S-17 , MPB1 , NNE , PPH
• Note: For research use only
• Application notes: Positive control: Mouse brain , A431 , H1299 , 293T
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of HeLa cells using ENO1 antibody

Immunohistochemical staining of human gastric tissue using ENO1 antibody

Western blot analysis of A431(Lane 1), H1299(Lane 2) using ENO1 antibody

Western blot analysis of A431(Lane 1), H1299(Lane 2) using ENO1 antibody