ENOA Antibody (C-term)

• Catalog Number: orb34267
• Category: Antibodies
• Description: Purified rabbit polyclonal antibody.
• Target: ENO1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Immunogen: Synthetic Peptide
• UniProt ID: P06733
• MW: 47169
• Tested applications: FC, IF, IHC-P, WB
• Dilution range: WB: 1:1000, FACS: 1:10-50, IF/ICC: 1:10-50, IHC-P: 1:10-50
• Clone Number: RB19995
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Anti-Alpha-enolase antibody, anti-2-phospho-D-glyc
• Research Area: Cancer Biology, Metabolism Research, Signal Transd
• Note: For research use only
• NCBI: NP_001419.1, NP_001188412.1
• Expiration Date: 12 months from date of receipt.

ENOA Antibody (C-term) western blot analysis in Jurkat, MCF-7, U251 cell line and mouse brain tissue lysates (35 ug/lane). This demonstrates the ENOA antibody detected the ENOA protein (arrow).

ENOA Antibody (C-term) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of ENOA Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

ENOA Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ENOA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.