ENC1 Antibody

• Catalog Number: orb1248564
• Category: Antibodies
• Description: ENC1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine
• Reactivity: Human, Rat
• Immunogen: The immunogen for this antibody is: TSVSHDKLPKVQC
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in Rat Ovary lysates (calculated MW of 66.1kDa according to Rat NP_001003401.1. Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse Brain for this particular batch.Immunohistochemistry:Paraffin embedded Human Adrenal Gland. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 70kDa
• Target: ENC1
• UniProt ID: O14682
• NCBI: NP_003624.1, NP_001243505.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: ENC1, ectodermal-neural cortex 1 (with BTB-like do
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in Rat Ovary lysates (calculated MW of 66.1kDa according to Rat NP_001003401.1. Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse Brain for this particular batch.Immunohistochemistry:Paraffin embedded Human Adrenal Gland. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248564 (1 ug/ml) staining of Rat Ovary lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248564 (5 ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.