ENC1 Antibody

• Catalog Number: orb12293
• Category: Antibodies
• Description: Goat polyclonal antibody to ENC1
• Target: ENC1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: TSVSHDKLPKVQC
• MW: 66.1
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml
• Application notes: WB: Approx 70kDa band observed in Rat Ovary lysates (calculated MW of 66.1kDa according to Rat NP_001003401.1. Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse Brain for this particular batch.. IHC: Paraffin embedded Human Adrenal Gland. Recommended concentration: 5µg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti CCL28 antibody, anti ectodermal-neural cortex
• Note: For research use only
• Entrez: 8507

orb12293 (1µg/ml) staining of Rat Ovary lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb12293 (5µg/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.