ENaC beta Antibody

• Catalog Number: orb67568
• Category: Antibodies
• Description: Rabbit Anti-Rat ENaC beta Polyclonal
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC, IP, WB
• Reactivity: Frog, Hamster, Human, Mouse, Rat
• Immunogen: Produced against the C-terminal tail (amino acids 617-638) of rat beta ENaC (antibody designation 3755-2)
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), IHC-P (1:100)
• Conjugation: Unconjugated
• MW: 87kDa
• Target: ENaC
• Entrez: 24767
• UniProt ID: P37090
• NCBI: NP_036780
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Alternative names: SCNN1B Antibody, Amiloride sensitive sodium channe
• Note: For research use only
• Application notes: 1 µg/ml of SPC-404 was sufficient for detection of beta-ENaC in 20 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

References

Ye Gao 1, Fei Cao 2, Xinyi Tian 1, Qianping Zhang 3, Congcong Xu 1, Bowen Ji 1, Ye-An Zhang 1, Linan Du 1, Jun Han 1, Li Li 1, Siyu Zhou 1, Yuqiang Gong 1, Binyu Ying 1, Fang Gao-Smith 4, Shengwei Jin Inhibition the ubiquitination of ENaC and Na,K-ATPase with erythropoietin promotes alveolar fluid clearance in sepsis-induced acute respiratory distress syndrome Biomed Pharmacother, 174, 116447 (2024)

PubMed: 38518606

Applications

WB

Reactivity

Rat

Han, Jun et al. Maresin Conjugates in Tissue Regeneration 1 improves alveolar fluid clearance by up-regulating alveolar ENaC, Na, K-ATPase in lipopolysaccharide-induced acute lung injury J. Cell. Mol. Med., 24, 4736-4747 (2020)

PubMed: 32160403

Immunohistochemistry analysis using Rabbit Anti-ENaC Polyclonal Antibody. Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody at 1:100. Secondary Antibody: FITC Goat Anti-Rabbit (green).

Western blot analysis of Rat kidney tissue lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody. Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody at 1:1000.

Western blot analysis of Mouse mpkCCD cell lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody. Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody at 1:1000.

Western blot analysis of Mouse kidney cortex showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody. Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody at 1:1000. Low-salt diet (lanes 1-4) compared to a high-salt diet (lanes 5-8).