ENaC beta Antibody

SKU: orb383764

Description

Mouse Anti-Rat ENaC beta Monoclonal IgG1

Research Area

Neuroscience, Pharmacology & Drug Discovery

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB (1:1000); IHC-P (1:150)
• Reactivity: Mouse
• Application Notes:
  A 1:1000 dilution was sufficient for detection of ENaC beta in 15 µg of Mouse whole kidney lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 7B8
• Immunogen: Synthetic peptide from the C-terminal of Rat ENaC beta (aa. 617-638)
• Target: ENaC beta
• Molecular Weight: 87kDa
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide. Storage buffer changes when conjugated.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Amiloride sensitive sodium channel subunit beta, Amiloride-sensitive sodium channel beta-subunit, Beta ENaC, Beta NaCH, ENaC beta, ENaCB, Epithelial Na(+) channel subunit beta, Epithelial Na+ channel beta subunit, Epithelial Na+ channel subunit beta, Epithelial sodium channel beta 2 subunit, Epithelial sodium channel beta 3 subunit, Nonvoltage gated sodium channel 1 beta subunit, Nonvoltage gated sodium channel 1 subunit beta, Nonvoltage-gated sodium channel 1 beta subunit, SCNEB, SCNN 1B, SCNN1B, Sodium channel nonvoltage gated 1 beta, Sodium channel nonvoltage gated 1 beta (Liddle syndrome)

Immunohistochemistry analysis using Mouse Anti-ENaC beta Monoclonal Antibody, Clone 7B8. Tissue: Kidney (cortex). Species: Mouse. Primary Antibody: Mouse Anti-ENaC beta Monoclonal Antibody at 1:150. Localization: Collecting duct principal cells. Magnification: 60X.

Western Blot analysis of Mouse Whole kidney homogenates showing detection of ~87kDa ENaC beta protein using Mouse Anti-ENaC beta Monoclonal Antibody, Clone 7B8. Lane 1: Molecular Weight Ladder (MW). Lane 2: Low-salt diet. Lane 3: Normal-salt diet. Load: 20 μg. Primary Antibody: Mouse Anti-ENaC beta Monoclonal Antibody at 1:1000. Predicted/Observed Size: ~87kDa.

Quick Database Links

Gene Symbol

ENaC beta

UniProt

P37090

NCBI Gene ID

24767

RefSeq (Protein):NP_036780